Osmolality as a concentration measurement method
for key buffers in bioprocessing
ABSTRACT: To control downstream bioprocessing, it is critical to monitor buffer properties such as conductivity and pH but, unfortunately, these measurements are not suitable for all buffers. Osmolality is a measure of solute concentration that could complement this system. In a study of several common buffers, osmolality was a more sensitive and reliable measurement than pH and conductivity across a range of buffer concentrations. Adding osmolality testing to downstream processing will provide a complete picture of buffer concentration and ensure product quality and yield.